The CFTRc has 4 state-of-the-art light microscopes to help answer several questions about CF research. These microscopes allow researchers to generate high quality images and quantitative data for high level publication, elevating research projects to the next level.

SOME OF THE SERVICES OFFERED:

• Live cell imaging
• Confocal imaging
• Differential Interference Contrast (DIC)
• Fluorescence Correlation Spectroscopy (FCS) to study ligand binding in solution and in cells
• Total Internal Reflected Fluorescence (TIRF)
• Quantitative imaging of protein and vesicle dynamics
• Measurement of intra and extracellular pH and Ca²⁺
   

^​OUR EQUIPMENT INCLUDES:

- the last generation of laser scanning confocal microscope (LSM780, Zeiss) mounted on an inverted Axio Observer fully motorized microscope; equipped with 3 high NA/DIC objectives (20x, 40xW, 63xOil)

- a Spinning-Disc/TIRF microscope (Zeiss) mounted on an inverted Axio Observer fully motorized microscope with an incubation unit; equipped with a 63x Oil objective designed for TIRF experiments

- two ratiometric wide-field imaging systems (PTI inverted microscope, Olympus and Axio Examiner upright, Zeiss).
 

HOW TO GET STARTED:

1. Fill out a Training Request Form
2. Set up a consultation time with one of our imaging experts
3. Fill out a Building Access Request Form to gain access to our microscopy rooms
4. Complete your training
    

For more information, visit the Advanced BioImaging Facility (ABIF) Website HERE.