Fiche Technique du Robot et de ses Modules

Avec ses 13 modules, notre robot SAGIAN est un véritable laboratoire automatisé pour le transfert de liquide ou de colonies, agitation, incubation, scellement de plaques, PCR, prise de données et de photos...

	Module Description
	Automated workstation (Biomek FX, Beckman Coulter) - Versatile liquid handler for 96- or 384-well plates. - Equipped with a 96-channel 200 μl head and a 8-independent channel head. - Pintools for 96-, 384 and 768 formats - Enclosed in an Iso class 5 cleanroom. - Different modules can be installed such as a magnetic bead unit, a filtration unit, a magnetic stirrer, an orbital shaker, a heating unit and a circulating reservoir.
	Incubator (Cytomat 6001, Kendro) - Accommodates up to 125 standard plates and 30 deep-well plates - Incubation from 10 to 50ºC. - CO2 supply possible for cell culture - Agar plates can be incubated (and manipulated) upside down to prevent condensation on lid.
	Plate reader (DTX880, Beckman Coulter) - For absorbance, luminescence, fluorescence intensity, fluorescence polarization and time-resolved fluorescence. - Heated chamber (from 4ºC above ambient up to 45ºC). - Reads from top or bottom of 6- to 384-well plates. - Current filter sets include 260, 340, 370, 405, 450, 485, 535, 595 and 620 nm for excitation and 535, 595 and 625 nm for emission. - Can perform endpoint, multiple wavelength, kinetic, or area scans.
	Digital Camera (EOS40D, Canon) - Enclosed for dark environment. - Two bulbs providing white light from top of the plate. - Polarized filters to prevent light reflection - Medium- to high-resolution pictures (JPEG, RAW format).
	Plate washer (ELx405 select, Biotek Instruments) - Dispenses from top and washes from bottom. - Flow rate is adjustable for sensitive assays.
	Carousel (Beckman Coulter) Accommodates up to 90 standard plates and 40 deep-well plates or tip boxes.
	Thermocycler (PTC-225 DNA engine tetrad, BioRad/MJ Research) 4-independent PCR units.
	Robotic arm (ORCA, Beckman Coulter) Slides on a 3-meter rail to move plates or labware between devices.
	Dispenser (Multidrop384, Titertek) 8-channel head to dispense 5 to 395 μl from a single or multiple reagent bottles
	Lidding station (Beckman Coulter) - Accommodates up to 6 lids. - Maintains the lid-plate association.
	Plate shaker (Beckman Coulter) Configured with 6-plate locations.
	Plate sealer (ALPS 300, from Abgene) Used to seal plates for storage or to prevent evaporation or contamination during incubation or shaking.
PICTURE	Tip lift and disposal unit (Beckman Coulter) Accommodates up to 96 200 μl tip boxes.

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