LSM1 and LSM2

LSM1LSM1 - Zeiss LSM510 Meta LSM2LSM2 - Zeiss LSM510 Meta

Microscope Base: Zeiss LSM510 on Axiovert 200M

LSM1: fully motorized

LSM2: motorized focus drive, manual x and y

 

 

Objectives

Position

Objective LSM1

Objective LSM2

1

10x/0.3 dry

10x/0.5 dry

2

20x/0.4 LD* dry

- empty -

programmed 10x/0.3 for maintenance only

3

20x/0.75 dry

25x/0.8 oil-Glyc-water**

4

- empty -

programmed for
40x water (see below)

40x/1.3 oil

5

40x/1.3 oil

63x/1.4 oil

6

63x/1.4 oil

100x/1.4 oil

available

40x/1.2 water

 

available

40x/0.6 LD* dry

 

* long working distance; positioned for 0.17mm cover slips, can be adjusted

** set for oil; can be adjusted for glycerol or water immersion

 

 

Confocal mode: Lasers, fluorescence emission filters (in addition to spectral ‘meta’ detector)

Laser lines

Emission filters LSM1

Emission filters LSM2

Typical fluorophore

Ch2

Ch3

Ch2

Ch3

405nm, Diode, Blue (LSM1)

blue and green emitters

 

LP420, LP475, LP505, BP420-480, BP470-500, BP475-525, BP505-530

orange, red, far-red emitters

 

LP505, LP560, LP650, BP500/20IR, BP505-550, BP530-600, BP560-615

blue and green emitters

 

LP475, LP505, BP475-525, BP500/20IR, BP505-530, BP530-600, BP505-550

orange, red, far-red emitters

 

LP505, LP530, LP560, LP585, BP560-615, BP585-615, LP650

DAPI

458nm, Argon, 30mW

CFP

477nm, Argon, 30mW

 

488nm, Argon, 30mW

FITC, GFP

514nm, Argon, 30mW

YFP

543nm, HeNe Green,1mW

Rhod., Cy3

633nm, HeNe Red, 5mW

Cy5

 

 

 

Wide-field fluorescence

Light source: SOLA Light Engine LED source

Filter set/cube

Excitation filter

Beam splitter

Emission filter

Typical fluorophore

Filter Set 00

BP 530-585

FT 600

LP 615

mCherry, AF594

Filter Set 13

BP 470/20

FT 493

BP 505-530

GFP

Filter Set 49

BP 390/18

FT 395

BP 445/50

DAPI

Filter Set 46 (LSM1 only)

BP 500/20

FT 515

BP 535/30

YFP