Objectives:
Position | Objective | Phase/DIC |
1 | 10x/0.3 dry | PH1 |
2 | 20x/0.7 oil-glyc-water* | DIC |
3 | 20x/0.5 dry | PH2 |
4 | 40x/1.25-0.75** oil | DIC |
5 | 63x/1.40-0.6** oil | DIC |
6 | 100x/1.40-0.7** oil | DIC |
* set for oil; can be adjusted for glycerol or water immersion
** must be adjusted for highest NA
Confocal mode: Lasers, fluorescence emission filters:
Lasers (all diode) | Emission filters | Typical fluorophore |
440nm, 15mW | 483/32 | CFP |
491nm, 25mW | 520/35 | GFP |
543/40 | YFP | |
561nm, 50mW | 624/40 | RFP/mCherry |
638nm, 30mW | 692/40 | FarRedFP |
| 593/40* | Cy3 |
| 645/75* | L-Cy5 |
*Available on demand
Wide-field fluorescence:
Light source: X-Cite120 metal-halide fluorescence lamp, 120W
Fluorescence filter cubes:
Filter set | Excitation filter | Dichroic mirror | Emission filter | Typical fluorophore |
CGFP | BP 436/20 | 455 | BP 480/40 | CFP |
GFP | BP 470/40 | 500 | BP 525/50 | GFP |
TX2 | BP 560/40 | 595 | BP 645/75 | Texas Red |