Technical data for SD2

SD2SD2 - Quorum WaveFX spinning disk on Leica DMI6000BMicroscope: Quorum WaveFX spinning disk confocal system, on a Leica DMI6000B inverted microscope, fully motorized, with a Hamamatsu EM-CCD camera; Live Cell Instruments Chamlide TC environmental control system

 

 

 

 

Objectives:

Position

Objective

Phase/DIC

1

10x/0.3 dry

PH1

2

20x/0.7 oil-glyc-water*

DIC

3

20x/0.5 dry

PH2

4

40x/1.25-0.75** oil

DIC

5

63x/1.40-0.6** oil

DIC

6

100x/1.40-0.7** oil

DIC

* set for oil; can be adjusted for glycerol or water immersion

** must be adjusted for highest NA

 

Confocal mode: Lasers, fluorescence emission filters:

Lasers (all diode)

Emission filters

Typical fluorophore

440nm, 15mW

483/32

CFP

491nm, 25mW

520/35

GFP

543/40

YFP

561nm, 50mW

624/40

RFP/mCherry

638nm, 30mW

692/40

FarRedFP

 

593/40*

Cy3

 

645/75*

L-Cy5

*Available on demand

 

Wide-field fluorescence:

Light source: X-Cite120 metal-halide fluorescence lamp, 120W

Fluorescence filter cubes:

Filter set

Excitation filter

Dichroic mirror

Emission filter

Typical fluorophore

CGFP

BP 436/20

455

BP 480/40

CFP

GFP

BP 470/40

500

BP 525/50

GFP

TX2

BP 560/40

595

BP 645/75

Texas Red