Standard Operating Procedures (SOPs)

Analgesia | Anesthesia | Surgery | Euthanasia | Experimental Guidelines | Animal Care Veterinary Care | Monitoring Log templateOccupational Health and Safety | Farm SOPs | Body Conditioning Score | Controlled Drugs Requirements | PHAC Biohazard info |  Draft SOPs |

Standard Operating Procedures (SOPs) provide a detailed description of commonly used procedures. SOPs offer investigators an alternative to writing detailed procedures in their protocol. Any deviation from the approved procedures must be clearly described and justified in the Animal Use Protocol application in Darwin. Approval of the protocol indicates approval of the deviation from the SOP for that project only. The following Standard Operating Procedures (SOPs) were created and/or revised by the Veterinary Care Subcommittee and/or the Occupational Health and Safety Subcommittee, and approved by the University Animal Care Committee.

If you have questions or have a recommendation to make, please send an animalcare [at] mcgill.ca (email) or talk to the Veterinarian in your area.

For Animal and Compliance Concerns, please consult the UACC Policy: PDF icon Policy on Animal and Compliance Concerns - June 2016

 


Draft SOPs for review by the UACC with a deadline of December 31, 2017.

Minor changes:

PDF icon SOP 101- Rodent Analgesia - Nov 2017

PDF icon SOP 102- Rabbit Analgesia - Nov 2017

PDF icon SOP 301- Rodent Euthanasia - Nov 2017

Substantial changes:

PDF icon SOP 532 - Vermin Control Program - Nov 2017

PDF icon SOP 602 - Rodent Health Monitoring Program - Nov 2017

PDF icon SOP 621- Rabbit Health Monitoring Program - Nov 2017

New SOP:

PDF icon SOP 703 - Use of Viral Vectors in Neuroscience - Rodents - Nov 2017

 


Analgesia: 

Note that most Analgesia SOPs were revised in Feb 2017

PDF icon SOP 101 - Rodent Analgesia - Feb 2017

PDF icon SOP 102 - Rabbit Analgesia - Feb 2017

PDF icon SOP 103 - NHP Analgesia - Feb 2017

PDF icon SOP 104 - Dog Analgesia - Feb 2017

PDF icon SOP 105 - Cat Analgesia - Feb 2017

PDF icon SOP 106 - Swine Analgesia - Oct 2016

PDF icon SOP 107 - Bird Analgesia - Feb 2017

PDF icon SOP 108 - Fish Amphibian and Reptile Analgesia - Feb 2017

PDF icon SOP 109 - Ruminant Analgesia - Feb 2017

 


 Anesthesia: 

PDF icon SOP 110 - Mouse Anesthesia - Oct 2016

PDF icon SOP 111 - Rat Anesthesia - Oct 2016

PDF icon SOP 112 - Hamster Anesthesia - Oct 2016

PDF icon SOP 113 - Guinea Pig Anesthesia - Oct 2016

PDF icon SOP 114 - Rabbit Anesthesia - Sept 2017

PDF icon SOP 115 - NHP Anesthesia - May 2017

PDF icon SOP 116 - Dog Anesthesia - Oct 2016

PDF icon SOP 117 - Cat Anesthesia - Oct 2016

PDF icon SOP 118 - Fish Aquatic and Amphibian Anesthesia - Oct 2016

PDF icon SOP 119 - Swine Anesthesia - Oct 2016

PDF icon SOP 120 - Anesthesia Protocol for Lung Physiology Measurements - Oct 2016

PDF icon SOP 121 - Bird Anesthesia - Oct 2016

 


Surgery:   

PDF icon SOP 201 - Rodent Surgery - Oct 2016

PDF icon SOP 202 - Rodent Stereotaxic Surgery - Oct 2016

PDF icon SOP 203 - Large Animal Surgery - Sept 2017

PDF icon SOP 204 - Xenopus Egg and Oocyte Collection - Oct 2016

PDF icon SOP 205 - Rodent Tumor Resection - Oct 2016

PDF icon SOP 206 - Rodent Ovariectomy - Oct 2016

PDF icon SOP 207 - Rodent Castration - Oct 2016

PDF icon SOP 208 - Rodent Mammary Fat Pad Injection - Oct 2016

 


Euthanasia:  

PDF icon SOP 301- Rodent Euthanasia - May 2017

PDF icon SOP 302 - Large Animal Euthanasia - Oct 2016

PDF icon SOP 303 - Fish and Amphibian Euthanasia - Oct 2016

PDF icon SOP 304 - Avian Euthanasia - Oct 2016

 


Experimental Guidelines:   

PDF icon SOP 401 - Controlled Drugs - Oct 2016 <- includes a log at the end 

PDF icon SOP 402 - Tissue Collection for Genotyping - Mice - Oct 2016  

PDF icon SOP 403 - Guidelines Blood Collection Volumes and Frequency - Sept 2017

Blood Volumes Annex

 PDF icon SOP 404 - Substance Administration - Sept 2017

PDF icon SOP 405 - Monoclonal Antibody Production - Mice - Oct 2016

PDF icon 406_-_polyclonal_antibody_production_-_rabbits_-_may2015.pdf

PDF icon SOP 407 - Polyclonal Antibody Production - Rats - Oct 2016

PDF icon SOP 408 - Water Control in Non-Human Primates - Oct 2016

PDF icon SOP 409 - Maintaining Clinical Records - Oct 2016

 PDF icon SOP 410 - Humane Intervention Points - Oct 2016

PDF icon SOP 412 - Humane Intervention Points for Aging Rodents - Oct 2016

PDF icon SOP 413 - Tissue Collection for Genotyping - Rats - Oct 2016

PDF icon SOP 415 - Humane Intervention Points for Rodent Cancer Models - Oct 2016

PDF icon SOP 416 - Social Defeat Model - Oct 2016

PDF icon SOP 417 - Food and Water Restriction in Rodents - May 2017

             

 This Monitoring Log is to be prepared by the Facility Animal Care Committee as per the approved Animal Use Protocol: File Monitoring log TEMPLATE - Oct 2017  

 

Tumor Volume

Metastatic Model Monitoring log  <- is a log

Mammary Gland Identification Mouse

Tumor Monitoring Log  <- is a log 

 


Animal Care:  

 PDF icon SOP 501 - Animal Transport and Use Outside of Animal Facilities - Oct 2016

PDF icon 502 - Environmental Enrichment for Mice - March 2016

PDF icon 503_-_environmental_enrichment_program_for_rats_-_may2015.pdf

PDF icon 504 - Environmental Enrichment for Rabbits - March 2016

PDF icon 505_-_environmental_enrichment_program_for_hamsters_-_may2015.pdf

PDF icon 506_-_environmental_enrichment_program_for_guinea_pigs_-_may2015.pdf

PDF icon 507_-_environmental_enrichment_program_for_nhps_-_may2015.pdf

PDF icon 508 - Rodent Husbandry - March 2016

PDF icon 509 - Rodent Daily Care 

511- Sentinel_Husbandry.pdf 

512- Contact_Sentinel_Husbandry.pdf

PDF icon SOP 513 Marmoset Husbandry

514- Marmoset_Daily_Care.pdf

515- Marmoset_Feeding.pdf

516- Marmoset_Cage_Changing 2013.pdf

517- Marmoset_Room_Sanitation.pdf 

PDF icon 518 - Transportation of NHPs to an Imaging Facility - March 2016 

PDF icon 519 - Aquatic Animal Husbandry

520- Dog_Husbandry 2013.pdf

521- Macaque_Husbandry 2013.pdf

522- Macaque_Daily_Care_2013.pdf

523- Rabbit_Husbandry.pdf

524- Rabbit_Daily_Care.pdf

525- Cat_Husbandry.pdf

526- Cat_Daily_Care.pdf 

PDF icon SOP 527 - Avian Husbandry - Oct 2016

PDF icon SOP 528 - Avian Daily Care - Oct 2016

529- Sheep_Husbandry_2013.pdf

 PDF icon 531 - Animal Acquisition Receiving and Acclimation - March 2016

PDF icon SOP 532 - Vermin Control Program - Oct 2016

533- Microbiological_Monitoring_Program 2013.pdf 

PDF icon 535 - Animal Facility Regulations - March 2016

PDF icon 536_-_procedure_room_maintenance_-_may2015.pdf

PDF icon SOP 537 - Working in Laminar Flow Hood or Animal Transfer Station or Biological Safety Cabinet - Oct 2016

538- Dog_Daily_Care_2013.pdf

PDF icon 539 - Maintenance of Passive Watering System - March 2016 

PDF icon 540_-_operating_room_maintenance_-_may2015.pdf


 

Veterinary Care: 

PDF icon SOP 601 - Rodent Veterinary Care Program - May 2017

602- Rodent Health Monitoring Program_2013.pdf

PDF icon SOP 603 - Rodent Quarantine Program - May 2017

604- Bioexclusion Levels  for Rodents.pdf

PDF icon SOP 605 -Rodent Import - May 2017 

PDF icon 607 - Long-Term Housing - March 2016

PDF icon 608- Mouse Breeding Colony Management - April 2016    

PDF icon 609 - Rat Breeding Colony Management - March 2016

PDF icon 610 - Testing of Biological Material - March 2016

611- Excluded_Pathogens - Rodent_Facilities_2013.pdf

PDF icon SOP 612 - Working with Immunodeficient Mice - May 2017

613- Working_with_Rodents_in_the_Imaging_Facility_2013.pdf

614- Guidelines_on_Wire-Bottom_Caging_for_Rodents_2013.pdf

PDF icon SOP 615 Post-Irradiation Care

PDF icon 616 - Rodent Identification - March 2016 

PDF icon 617 - Treatment of Pinworms in Rodent Colonies - March 2016 

PDF icon SOP 618 - Treatment of Fur Mites in Rodent Colonies - Sept 2017

621- Rabbit_Health_Monitoring_Program_2013.pdf

622- Marmoset_Health_Monitoring_Program_2013.pdf

623- Marmoset_Breeding_Colony_Management 2013.pdf

PDF icon SOP 624 - Cat Breeding Program - Sept 2017

PDF icon SOP 625 - Cat Conditioning Program - May 2017

626- Macaque_Health_Monitoring_Program_2013.pdf

627- Non-Human_Primate_Quarantine_Program_2013.pdf

 


 

Occupational Health and Safety: 

701- Macaque_Related_Injury 2013.pdf

PDF icon 702 - Animal Related Injury - March 2016 

PDF icon 705 - Animal Biosafety Level 2 - Rodents - March 2016 

PDF icon 706 - Use of Hazardous Agents in Rodents - General - March 2016 

PDF icon 707 - Occupational Health Program - March 2016  

PDF icon 708 - Use of Streptozotocin in Rodents - March 2016 

PDF icon SOP 709- Waste Anesthetic Gas Scavenging - Sept 2017 

710- Personal Protective Equipement Requirements.pdf 

PDF icon 711 - Use of TNBS in Rodents - March 2016

PDF icon 712 - Use of Azoxymethane in Rodents - March 2016 

PDF icon 713 - Use of BBN in Rodents - March 2016   

PDF icon 714 - Use of MPTP in NHPs - March 2016              

PDF icon 715 - Use of Cisplatin in Rodents - March 2016

PDF icon 716 - Use of Doxorubicin in Rodents - March 2016

PDF icon Allergy Prevention - March 2016

PDF icon Tuberculosis Factsheet - March 2016

 

 


Farm SOPs:

MAC FACC Web site

 


 

Body Condition Score:

Body Condition Score (BCS)

Body Condition Score Outline Mice
Body Condition Score Outline For Rats

Of interest: Article Body Condition Score for Rats

 


Requirements on Record Keeping, Storage and Disposal of Controlled Drugs:

 

The storage conditions and physical security for the controlled substance must meet the requirements of the "Directive on Physical Security Requirements for Controlled Substances", available on the Health Canada website: http://www.hc-sc.gc.ca/hc-ps/substancontrol/substan/securit-eng.php
For scientific exemptions, the required security level is Security Level - 1 and the maximum licensee holdings is $5000.  Please note that each researcher must have his or her own storage.

The conditions of record keeping and disposal is outlined in the exemption letter as follows:

The researcher is required to maintain records with respect to his/her possession and use of the controlled substance in accordance with the applicable regulations.  The researcher shall furnish such information respecting such controlled substances as the Minister may requires and shall permit access to the records required to be kept by these Regulations. Specifically, the researcher must keep and retain for a period of two years from the making of such record, the following information:

•        the kind, date and quantity of any controlled substance purchased or received;
•        the name and address of the person from whom the controlled substance was received; and particulars of the use to which the controlled substance was put.

The researcher is responsible for the destruction of any unused or expired controlled substance.  The destruction must be witnessed by a member of his/her research staff who is working on the same research project as specified in the exemption, and who works under the researcher’s direction and control.  The method of destruction used must alter or denature the controlled substance in such a way as to make it non-recoverable and thus make their consumption improbable or impossible. The researcher is required to keep and retain for a period of two years from the date of the making of the record, the following information:

•        the name, strength per unit, and quantity of any controlled substance to be destroyed;
•        the date of destruction; and
•        the reason for destruction.

Immediately following the destruction, the researcher and the witness are required to sign and print their names on a joint statement indicating that they witnessed the destruction and that the controlled substance destroyed has been altered or denatured to such an extent that its consumption has been rendered impossible or improbable.

The researcher shall make such records available to the Minister or an inspector upon request.

 


 

PHAC Biohazard Information

The Public Health Agency of Canada has implemented since December 2015, new regulations pertaining to the use of Human Pathogens and Toxins. Background information can be found on their site and details on the regulation here

In order to work with Biohazardous Materials in a laboratory setting, researchers must complete an “Application to Use Biohazardous Materials”.  These applications are reviewed and approved by McGill's Environmental Health and Safety Office (not the Animal Care Committee). Approval of the Application means that the researcher is compliant with the Biosafety Program which includes lab inspections and training.  

Useful links (these two are also available as free Apps for a smart phone or tablet as well):