Total Internal Reflection Fluorescence (TIRF) microscope for looking within ~100 nm of the microscope slide. Specifically well suited for endocytosis, exocytosis, adhesion and membrane protein/lipid studies.
Features:
- Total Internal Reflection Fluorescence (TIRF)
- Variable pinhole Spinning Disk Confocal
- Rapid simultaneous two colour imaging on EMCCD
- Environmental Control for live cell imaging
Light Sources:
- Halogen Lamp for Transmitted Light
- Wide Field Microscopy - X-Cite 120 LED (370-700nm), 100W
- Diode Laser - 488nm - 100 mW - with FLCRtm control system
- Diode Laser - 561nm - 100 mW
- Diode Laser - 642 nm - 110 mW
Objectives:
- 10X N-PLAN, NA=0.25
- 20X HC PLAN APOCHROMAT, NA=0.70,
- 63X HCX PLAN APOCHROMAT, NA=1.47, OIL, Corr, TIRF
Fluorescence Filter Cubes:
- EGFP (ET 525/50nm)
- mCherry (ET 620/60nm)
- EGFP/mCherry Dual Cube
Fluorescence Probes:
- EGFP
- mCherry
- Cy5/IR-FP
Detectors:
- Hamamatsu ImageEMX2 EM-CCD Cameras
Additional Features:
- DIC Optics
- High resolution sequential multi-colour imaging
Location:
- Located in Bellini Building, Room 147.