We are looking for a Research Assistant to perform work related to monoclonal antibody (Mab) generation, characterization, and functional testing. The position will be to support a project where the generation and testing of Mab is required. We are looking for someone with prior tissue culture expertise and experience of Mab generation and subsequent characterization.
The candidate must be able to perform analytical and preparative procedures required for the development and analysis of monoclonal antibodies; characterize antibodies by designing and optimizing experiments, interpreting data, and selecting the best possible products to develop. She/he must have the capacity to utilize established procedures and techniques and the ability to investigate for new protocols/procedures and techniques to assist with the validation of novel Mabs as potential biomarkers of early Alzheimer’s pathology. He/she will work under the direct supervision of Prof. Cuello.
- Collaborate in the design of epitopes for immunization
- Prepare and/or order peptides and peptide conjugates to proteins.
- Perform immunizations and periodic bleeding in rodents
- Investigate the presence of desired rodent polyclonal antibodies in immunized rodents
- Preparing primary tissue culture for cell fusion
- Perform the Kohler-Milstein Protocol to generate Monoclonal Antibodies, with the Lab’s modifications
- Handling of mice and rats, process their tissue for immunohistochemistry or neurochemistry
- Perform ELISAs and Western blot analysis
- Use of light and confocal microscopes
Master’s Degree in Sciences (with appropriate experience.)
Other Qualifying Skills and/or Abilities:
Be highly organized, with proven experience in a laboratory environment. Have good interpersonal skills and be able to interact productively and accurately with other team members. Should be able to: exercise scientific judgment and independence. Plan and execute research strategies in a goal-oriented fashion. Troubleshoot; generate, analyze, and interpret data. Contribute ideas, novel techniques, or resources to advance goals. Maintain accurate and detailed records of lab experiments.
The working hours for this position is from Monday to Friday. The location of this position is at the McIntyre Medical Building, 3655 Promenade Sir William Osler, Montreal.
How to Apply:
Please send your cover letter, CV and contact information for two referees by email to : adriana.ducatenzeiler [at] mcgill.ca.
McGill University is committed to equity in employment and diversity. It welcomes applications from indigenous peoples, visible minorities, ethnic minorities, persons with disabilities, women, persons of minority sexual orientations and gender identities, and others who may contribute to further diversification.