Event
Chemical Society Seminar: Joshua A. Kritzer - Cell Penetration Profiling for Biotherapeutics
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Abstract:
Cell penetration is a major obstacle for developing peptide, protein and nucleic acid therapeutics. Many commonly used techniques for measuring cell penetration are qualitative, and most cannot distinguish cytosolic material from material trapped at the cell surface or in endosomes. We have devised a new technique, the chloroalkane penetration assay (CAPA), that measures penetration to the cytosol in a high-throughput, quantitative manner. We are using CAPA in a wide range of applications, including: determining structure-penetration relationships for peptide therapeutics, quantitating penetration into different subcellular organelles and compartments, and measuring cytosolic penetration for a wide variety of druglike macrocycles, peptides, oligonucleotides, and proteins.