Technical data for Deco1

Deco1Deco1 - Leica DM6000B upright microscopeMicroscope: Leica DM6000B upright microscope, motorized stage in z, motorized optics, Hamamatsu ORCA-EM EM-CCD camera, acquisition software Volocity

 

 

 

 

Objectives

Position

Objective

1

10x/0.3 dry

2

 

3

 

4

40x/1.25 oil

5

63x/1.4 – 0.60* oil

6

 

* variable NA; make sure to check that the aperture is all the way open before imaging

Wide-field fluorescence
Light source: Exfo metal halide lamp (Lumen Dynamics)

Filter set/cube

Excitation filter

Beam splitter

Emission filter

Typical fluorophore

Filter Set A4

BP 360/40

400

BP 470/40

DAPI

Filter Set L5

BP 480/40

505

BP 527/30

GFP, AF488, FITC

Filter Set Y3

BP 545/40

565

BP 610/75

AF546, 555, Cy5

Filter Set YFP

BP 500/20

515

BP 535/30

YFP

Filter Set CFP

BP 436/20

455

BP 480/40

CFP