Microscope Base: Zeiss LSM510 on Axiovert 200M
LSM1: fully motorized
LSM2: motorized focus drive, manual x and y
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Objectives |
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Position |
Objective LSM1 |
Objective LSM2 |
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1 |
10x/0.3 dry |
10x/0.5 dry |
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2 |
20x/0.4 LD* dry |
- empty - programmed 10x/0.3 for maintenance only |
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3 |
20x/0.75 dry |
25x/0.8 oil-Glyc-water** |
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4 |
- empty - programmed for |
40x/1.3 oil |
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5 |
40x/1.3 oil |
63x/1.4 oil |
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6 |
63x/1.4 oil |
100x/1.4 oil |
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available |
40x/1.2 water |
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|
available |
40x/0.6 LD* dry |
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* long working distance; positioned for 0.17mm cover slips, can be adjusted
** set for oil; can be adjusted for glycerol or water immersion
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Confocal mode: Lasers, fluorescence emission filters (in addition to spectral ‘meta’ detector) |
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Laser lines |
Emission filters LSM1 |
Emission filters LSM2 |
Typical fluorophore |
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Ch2 |
Ch3 |
Ch2 |
Ch3 |
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405nm, Diode, Blue (LSM1) |
blue and green emitters
LP420, LP475, LP505, BP420-480, BP470-500, BP475-525, BP505-530 |
orange, red, far-red emitters
LP505, LP560, LP650, BP500/20IR, BP505-550, BP530-600, BP560-615 |
blue and green emitters
LP475, LP505, BP475-525, BP500/20IR, BP505-530, BP530-600, BP505-550 |
orange, red, far-red emitters
LP505, LP530, LP560, LP585, BP560-615, BP585-615, LP650 |
DAPI |
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458nm, Argon, 30mW |
CFP |
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477nm, Argon, 30mW |
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488nm, Argon, 30mW |
FITC, GFP |
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514nm, Argon, 30mW |
YFP |
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543nm, HeNe Green,1mW |
Rhod., Cy3 |
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633nm, HeNe Red, 5mW |
Cy5 |
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Wide-field fluorescence |
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Light source: SOLA Light Engine LED source |
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Filter set/cube |
Excitation filter |
Beam splitter |
Emission filter |
Typical fluorophore |
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Filter Set 00 |
BP 530-585 |
FT 600 |
LP 615 |
mCherry, AF594 |
|
Filter Set 13 |
BP 470/20 |
FT 493 |
BP 505-530 |
GFP |
|
Filter Set 49 |
BP 390/18 |
FT 395 |
BP 445/50 |
DAPI |
|
Filter Set 46 (LSM1 only) |
BP 500/20 |
FT 515 |
BP 535/30 |
YFP |