Technical data for OMX

OMXOMX - Applied Precision OMX V.4

Microscope: Applied Precision V4 Blaze OMX system with TIRF option, two Evolve EM-CCD cameras, live cell chamber, DIC optics, in a light-proof enclosure with HEPA-filter, acquisition and analysis workstations

 Objectives (only one can be installed at a time):

Objective

100x/1.4 oil

Olympus, API Certified U-PLANAPO

100x/1.49 TIRF oil

Olympus, API Certified U-PLANAPO

 

Structured Illumination (SIM) and localization microscopy (DLM) modes:

Lasers (all diode)

Emission filters

Typical fluorophore

405nm, 100mW

BP 436/31 (420-451nm)

DAPI

488nm, 200mW

BP 528/48 (504-552nm)

GFP, AF488

568nm, 100mW

BP 609/37 (591-619nm)

mCherry, AF568

642nm, 300mW

BP 683/40 (663-703nm)

AF647

 

 Conventional wide-field fluorescence:

Light source: 6-color InsightSSI (solid state illumination) module

Fluorescence filters:

Excitation Wavelengths

Emission filters

Typical fluorophore

381-399nm

BP 436/31 (420-451nm)

BFP, DAPI

426-450nm

BP 478/35 (460-495nm)

CFP

461-489nm

BP 528/48 (504-552nm)

GFP, FITC, Alexa 488

505-515nm

BP 541/22 (530-552nm)

YFP, Alexa 532

563-588nm

BP 609/37 (590-618nm)

mCherry, Alexa 568

621-643nm

BP 683/40 (663-703nm)

Cy5, Alexa 647, 635, 660