Technical data for SD3

SD3SD3 - Quorum WaveFX-X1 spinning disk on Leica DMI6000BMicroscope: Quorum WaveFX-X1 spinning disk confocal system, on a Leica DMI6000B inverted microscope, fully motorized, with an ASI MS-2000 piezo stage, and two Hamamatsu “ImagEM” EM-CCD cameras; Live Cell Instruments Chamlide TC environmental control system

 

 

 

 

Objectives:

Position

Objective

Phase/DIC

1

10x/0.4 dry

HC PL APO; 506284

 

2

20x/0.7 dry

HC PLAN APO; 506166

DIC

3

40x/0.85 dry corr

0.11-0.23 HCX PL APO;

506294

DIC

4

40x/1.25-0.75** oil lBL

HCX PL APO; 506253

DIC

5

63x/1.40-0.60** oil lBL

HCX PL APO; 506192

DIC

6

Micropoint alignment device

 

** must be adjusted for highest NA

 

Confocal mode: Lasers, fluorescence emission filters:

Lasers (all diode)

Emission filters
(main camera, wheel 1)

Emission filters
(secondary camera)

Typical fluorophore

446nm, 40mW

HQ 470/40

HQ 470/40

CFP

491nm, 50mW

ET 525/50

ET 525/50

GFP

ET 540/40

 

YFP

568nm, 50mW

FF 593/40

FF 593/40

OFP (orange)

ET 620/60

 

RFP/mCherry

643nm, 110mW

ET 700/75

 

FarRedFP

Additional filters for main camera (wheel 2, orange and red emitters): ET 620/60, HQ 650/100, ET 700/75, HQ 600 LP, HQ 655 LP

 

Wide-field fluorescence:

Light source: X-Cite120Q metal-halide fluorescence lamp, 120W

Fluorescence filter cubes, Leica:

Filter set

Excitation filter

Dichroic mirror

Emission filter

Typical fluorophore

CGFP

BP 436/20

455

BP 480/40

CFP

GFP

BP 470/40

500

BP 525/50

GFP

TX2

BP 560/40

595

BP 645/75

Texas Red