Objectives | |
Position | Objective |
1 | 10x/0.3 dry |
2 |
|
3 |
|
4 | 40x/1.25 oil |
5 | 63x/1.4 – 0.60* oil |
6 |
|
* variable NA; make sure to check that the aperture is all the way open before imaging |
Wide-field fluorescence | ||||
Filter set/cube | Excitation filter | Beam splitter | Emission filter | Typical fluorophore |
Filter Set A4 | BP 360/40 | 400 | BP 470/40 | DAPI |
Filter Set L5 | BP 480/40 | 505 | BP 527/30 | GFP, AF488, FITC |
Filter Set Y3 | BP 545/40 | 565 | BP 610/75 | AF546, 555, Cy5 |
Filter Set YFP | BP 500/20 | 515 | BP 535/30 | YFP |
Filter Set CFP | BP 436/20 | 455 | BP 480/40 | CFP |