Laboratory Animal Biomethodology Workshops

The Canadian Council on Animal Care (CCAC) has made training mandatory starting January 2003. McGill University’s Animal Care Committees started implementing the training program fully in 2004. The approval of new Animal Use Protocols as well as renewals are conditional on all personnel having the necessary training. Each individual listed in the personnel section needs to have passed the theory course and, if handling live animals, have at least registered for the practical course specific to the species and procedures involved in the project.

To assist researchers in meeting these training requirements, the Comparative Medicine & Animal Resources Centre (CMARC) offers a variety of biomethodology workshops for laboratory animals. Our Veterinary Care team has extensive knowledge and expertise with numerous techniques and procedures in several species.

The practical training has been subdivided into modules to tailor the training to each individual’s needs.

We encourage workshop participants to only enroll in the training sessions that are relevant to their research protocol and to complete their training as close as possible to the start of the research project. 

Module 1 – Introduction to the Laboratory Mouse/Rat

All individuals working with laboratory rodents must complete this module.

 
Course Content:

  • Species overview
  • Handling and restraint
  • Identification
  • Sexing
  • Tissue sampling for genotyping
  • Euthanasia: isoflurane anesthesia followed by CO2, CO2 euthanasia
  • Terminal blood collection: intracardiac puncture
     

NOTE:  During this workshop, you may be exposed to waste anesthetic gases (isoflurane). If you are currently pregnant, please consult your physician or McGill University's Environmental Health and Safety department before enrolling. 

 

Course Handouts

PDF icon Mouse Module 1 Handout

PDF icon Rat Module 1 Handout

Module 2 - Substance Administration and Blood Collection (Mouse/Rat)

***Module 1 is a prerequisite

Participants are only required to complete training for the procedures listed in their Animal Use Protocol.

 
Course Content:

  • Subcutaneous injection
  • Intraperitoneal injection
  • Intramuscular injection (rat only)
  • Blood collection: lateral saphenous vein

              NOTE: training for other blood collection methods available as specialized workshops

 

Course Handouts

PDF icon Mouse Module 2 Handout

PDF icon Rat Module 2 Handout

Module 3 – Rodent Anesthesia and Analgesia (Mouse/Rat/Hamster/Gerbil)

***Modules 1 and 2 are prerequisites

This module is targeted to participants who will be anesthetizing rodents.

 
Course Content

  • Pain recognition and assessment
  • Analgesia
  • Injectable anesthesia (video)
  • Gas anesthesia
  • Monitoring during anesthesia
  • Recovery
     

NOTE:  During this workshop, you may be exposed to waste anesthetic gases (isoflurane). If you are currently pregnant, please consult your physician or McGill University's Environmental Health and Safety department before enrolling. 

 

Course Handout

PDF icon Rodent Module 3 Handout

Module 4 – Rodent Surgery and Aseptic Technique (Mouse/Rat/Hamster/Gerbil)

***Modules 1, 2 and 3 are prerequisites

This module is exclusively for participants who will be performing recovery surgeries on rodents; it is composed of 3-4 training sessions

 
Course Content:

  • Basic principles of rodent surgery
  • Aseptic technique
  • Wound closure
  • Pre-operative considerations
  • Intra-operative procedures
  • Post-op care
     

To register for this workshop, workshop.cmarc [at] mcgill.ca (subject: Surgery%20Training%20Information) (contact us).

NOTE:  During this workshop, you may be exposed to waste anesthetic gases (isoflurane). If you are currently pregnant, please consult your physician or McGill University's Environmental Health and Safety department before enrolling. 

 

Module 5 – Rodent Breeding Colony Management (Mouse/Rat)

***Module 1 is a prerequisite

This module is targeted to participants who will be breeding rodents.

 
COMING SOON

Module 6 – Intravenous Injection Mouse

***Module 1 is a prerequisite

This module is specifically for participants who will be performing intravenous injections in mice as part of their research project.

 

Course Handout

PDF icon Mouse Intravenous Injection Handout

 

For intravenous injection training in rats, workshop.cmarc [at] mcgill.ca (subject: Rat%20Intravenous%20Injection%20Training) (contact us).

 

 

If you have any questions, feel free to workshop.cmarc [at] mcgill.ca (contact us).