Targeted quantitation of covalently modified proteins

Mass Spectrometry Seminar, with Special guest speaker: Dr. Lekha Sleno, Department of Chemistry, Université de Québec à Montréal.

Dr Sleno's lab is interested in identifying and quantifying reactive metabolite modifications on proteins. She will present their recent work on the quantitation of acetaminophen modified serum albumin in both a rat model and in an acute liver failure cohort, while explaining the steps needed to develop a method to measure exact amounts of modified protein. They have used a surrogate protein level standard to account for all losses during sample prep and yield a surrogate calibration curve for the modified analyte peptide. They have then applied the use of this surrogate standard to study acetaminophen modifications on different glutathione-S-transferases.

ABOUT THE SPEAKER:

Lekha Sleno received her PhD in chemistry from Dalhousie University in 2006, where her work involved small molecule mass spectrometry. She then completed two postdoctoral fellowships at the University of Geneva in Switzerland (in pharmaceutical analytical chemistry) and the University of Toronto (at the Donnelly CCBR in proteomics research). She is currently associate professor in the chemistry department at UQAM (Université du Québec à Montréal). Her research interests include bioanalytical mass spectrometry applied to metabolomics and covalent binding of reactive drug metabolites.

This talk is opened to anyone interested in learning more about Mass Spectrometry and its applications will obtain a great deal from these monthly workshops.

For more information:anca.baesu [at] mail.mcgill.ca ( Anca Baesu);stephane.bayen [at] mcgill.ca ( Dr Stéphane Bayen)