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iPSC Culture Protocol Videos
Watch our iPSC culture protocol videos to learn how to culture iPSCs and iPSC-derived neural cells.
English, French, Portuguese, and Spanish videos are available now, with more languages coming soon!
At the EDDU, we developed a method with microfabricated disk (EB disk-948 from eNUVIO) to scale up the generation of hMOs (human midbrain organoids). This opens up the possibility to generate larger numbers of hMOs, in a manner that minimizes the amount of labor required, while decreasing variability and maintaining the viability of these hMOs over time.
This video will describe the methodology to generate hMOs from a single EB-Disk containing 948 microwells.
The Neuro’s EDDU is also pleased to share our updated protocol for generating dopaminergic neurons from iPSCs through the use of a Microfabricated EB Disk. You can access the protocol here: https://zenodo.org/record/8256637
Latest Publications
To access all of the EDDU's publications, please visit our Community and Resource Hub.
Homozygous ALS-linked mutations in TARDBP/TDP-43 lead to hypoactivity and synaptic abnormalities in human iPSC-derived motor neurons
February 2024
Sarah Lépine et al.
The use of a SOX10 reporter toward ameliorating oligodendrocyte lineage differentiation from human induced pluripotent stem cells
March 2024
Valerio E. C. Piscopo et al.
Preprints
Early nuclear phenotypes and reactive transformation in human iPSC-derived astrocytes from ALS patients with SOD1 mutations
October 2023
Vincent Soubannier et al.
Transcriptome-based screening in TARDBP/TDP-43 knock-in motor neurons identifies the NEDD8-activating enzyme inhibitor MLN4924
January 2024
Sarah Lépine et al.
How to Access iPSCs
At the EDDU we have a catalogue of iPSC lines from healthy individuals and from patients with neurological disorders that can be freely accessed for a small fee. Our cells are for experimental use only.
How to access the iPSC catalogue:
- Click “Open data access” as login
- Select at the top-left “Open data” tab
- Click “iPSC catalogue”
To access the cells, please contact cbig.mni [at] mcgill.ca (C-BIG). Click here for further instructions on accessing cells.
To learn more about how the cells are made or for general inquiries, contact us at neuroeddu.mni [at] mcgill.ca.
Visit our Community and Resource Hub
To access the EDDU’s Open Science resources including our publications, presentations, protocols, and software please visit our Community and Resource Hub.
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