Trypanosoma cruzi possesses hundreds of genes associated with pathogenesis. The extent and organization of this diverse gene repertoire, expression, and role in infection remain unclear. Using accurate long-read sequencing and chromatin conformation capture, we assembled T. cruzi Sylvio X10 strain chromosomes from telomere-to-telomere. The genome provides accurate organization of multigene family genes, confirming their distribution in expanded clusters or scattered throughout the chromosomes....

Host specificity is a critical feature of the survival and proliferation of parasites. In the context of interactions with the host, numerous mechanisms have been identified, particularly in parasitic helminths, that enable manipulation of the host immune system to enhance their own survival. The evolutionary history of these interactions often results in hosts becoming disease-tolerant or asymptomatic, even when burdened with a high number of worms, until a disruption in the host's immune...