Guidelines
The HTS staff is responsible for the management of the chemical libraries, for the operating and maintaining the robotics and readers required during an HTS screen. The HTS staff will provide screeners with training in the use of some equipment such as microplate dispenser and plate readers. Only screening facility personnel are permitted to handle compound libraries, and thus they perform all compound transfers from library plates into assay plates. Generally, screeners perform their own assays and provide their own supplies including assay plates and reagents.
It is our philosophy that post-doctoral fellows or graduate students will work together with the HTS staff in the execution of their particular screen. Part of our mandate is to also train students.
Scientists interested in performing a screen are welcomed to follow the guidelines, fill out the application form and contact us for further details.
McGill University Life Sciences Complex HTS Review Committee would review all HTS applications in consultation with the investigators involved. Once a HTS application is approved the investigator will be notified of the starting date of the screen and its probable duration.
Request form [.doc]
Instructions for requesting cherry picks
Cherry pick request can be made during the screening process. Generally, users are permitted to request cherry picks for up to 0.3% of the total compounds screened. For example, if you screened 50,000 compounds, you can request up to 150 (0.3%) cherry picks. Screeners with a high "hit" rate may choose their cherry picks by examining the structures of their screening positives.
Due to the limited amount of compound stocks for our libraries, please note that we may supply small amounts of compound for hit confirmation (1 ul of 10 mM stock) from part of our collection. If you require more than 1 ul of compound or in the case of low compound stocks in our collection, you may directly order compounds from the original source or other compound suppliers.
For anyone requesting cherry picks a request from needs to be filled out. All these annotated results will be stored in our database. This information is essential to us since it documents the results of each assay performed at our facility. Therefore, no cherry picks will be released without filling out the cherry pick request form in Microsoft Excel (please follow the example in the cherry pick application form).
Cherry pick requests files must include all positives annotated as a W, M, or S (weak, medium or strong) or by other criteria, e.g. by how many times from the standard deviation. Please provide the criteria you use to define your positive. The columns should be McGill plate ID, Well (please use the format in the example), Positive, Cherry Pick, and Comment. The file must contain the number of cherry picks being requested and the total number of compounds screened to ensure compliance with the "0.3% Rule". Place a C in the Cherry Pick column for those wells that you decide to cherry pick. Enter a comment for all positives that you decide not to cherry pick.
Cherry pick form [.doc]
pSM2 Retroviral shRNAmir Libraries
To Obtain Clones:
Clones (streaked plates from bacterial stocks) from the libraries are available for McGill investigators.
To request specific clones, identify the clones using the Open Biosystems Query page using the RefSeq accession number (RefSeq accession # will start with NM or XM) (or gene name) for the desired gene. Then sort the list by species (human or mouse) and vector (pSM2). Click on the Oligo ID and scroll down to location, please note the plate and well location as well as the unique clone ID.
Email the HTS/HCS Facility staff to request clones using Open Biosystems unique clone ID (v2HS_# clone ID for human; v2MM_# clone ID for mouse), plate and well location.
At the mutually agreed time bring your bacterial plates for streaking to the McIntyre Building room 914. (Plates must contain 25 ug/mL of chloramphenicol and 25 ug/mL of kanamycin.)
Human shRNA library:
- RHS3899-CSHL Hs shRNAmir 1.3 Plates SH2001-SH2048
- RHS3900-CSHL Hs shRNAmir 1.4 Plates SH2049-SH2100
- RHS3901-CSHL Hs shRNAmir 1.5 Plates SH2101-SH2150
- RHS3902-CSHL Hs shRNAmir 1.6 Plates SH2151-SH2200
- RHS3903-CSHL Hs shRNAmir 1.7 Plates SH2201-SH2250
- RHS3904-CSHL Hs shRNAmir 1.8 Plates SH2251-SH2300
- RHS3905-CSHL Hs shRNAmir 1.9 Plates SH2301-SH2350
- RHS3906-CSHL Hs shRNAmir 1.10 Plates SH2351-SH2400
- RHS3959-CSHL Hs shRNAmir 1.11 Plates SH2401-SH2450
- RHS3960-CSHL Hs shRNAmir 1.12 Plates SH2451-SH2500
- RHS3961-CSHL Hs shRNAmir 1.13 Plates SH2501-SH2550
- RHS3962-CSHL Hs shRNAmir 1.14 Plates SH2551-SH2600
- RHS3963-CSHL Hs shRNAmir 1.15 Plates SH2601-SH2650
Mouse shRNA library:
- RMM1766-CSHL Mm shRNAmir 2.3 Plates SM2001-SM2018
- RMM1766-CSHL Mm shRNAmir 2.4 Plates SM2019-SM2050
- RMM1766-CSHL Mm shRNAmir 2.5 Plates SM2051-SM2100
- RMM1766-CSHL Mm shRNAmir 2.6 Plates SM2101-SM2150
- RMM1766-CSHL Mm shRNAmir 2.7 Plates SM2151-SM2200
- RMM1766-CSHL Mm shRNAmir 2.8 Plates SM2201-SM2250
- RMM1766-CSHL Mm shRNAmir 2.9 Plates SM2251-SM2300
- RMM1766-CSHL Mm shRNAmir 2.10 Plates SM2301-SM2350
- RMM1766-CSHL Mm shRNAmir 2.11 Plates SM2351-SM2400
- RMM1766-CSHL Mm shRNAmir 2.12 Plates SM2401-SM2450
- RMM1766-CSHL Mm shRNAmir 2.13 Plates SM2451-SM2500
- RMM1766-CSHL Mm shRNAmir 2.14 Plates SM2501-SM2550
- RMM1766-CSHL Mm shRNAmir 2.15 Plates SM2551-SM2600
- RMM1766-CSHL Mm shRNAmir 2.16 Plates SM2601-SM2650