Total Internal Reflection Fluorescence (TIRF) microscope for looking within ~100 nm of the microscope slide. Specifically well suited for endocytosis, exocytosis, adhesion and membrane protein/lipid studies.
Features:
- Total Internal Reflection Fluorescence (TIRF)
- Spectral Diskovery system based on a Leica microscope
- Rapid dual colour imaging
- Live cell imaging chamber
Light Sources:
- Halogen Lamp for Transmitted Light
- X-Cite 120 LED (370-700nm), 100W
- Diode Laser - 488nm with FLCRtm control system
- Diode-Pumped Solid State (DPSS) Laser - 561nm
Objectives:
- 20X HC PLAN APOCHROMAT, NA=0.7, DIC
- 63X PLAN APOCHROMAT, NA=1.47, OIL, DIC
Fluorescence Cubes:
- EGFP (ET 525/50nm)
- mCherry (ET 620/60nm)
- EGFP/mCherry Dual Cube
Detectors:
- Hamamatsu Camera
-
EMCCD Digital Camera
- ImagEMX2
Additional Features:
- DIC Optics
- High resolution sequential multi-colour imaging
Location:
- Located in Bellini Building, Room 147.