Dynamic light scattering: Brookhaven Instrument NanoBrook Omni
Contact: violeta.toader [at] mcgill.ca (Violeta Toader) - Location: OM 21
The NanoBrook Omni particle size and zeta potential analyzer incorporates all you need for fast, routine, sub-micron measurements of size and zeta potential.
The measurements are based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, and based on Doppler velocimetry (electrophoretic light scattering or ELS) for zeta potential measurement. The instrument also has the capacity of zeta potential detection by Phase Analysis Light Scattering (PALS) measurements for samples with low mobilities.
The instrument has also an autotitrator unit with a volume of 20 mL. With 4-pumps, a pH probe and the possibility of temperature control, this accessory allows user to measure the variation of zeta potential of their sample with concentration of an additive (such as acids, bases, surfactants, dispersing agents, salts, etc.)
Features at a glance
Sizing
- Rapid and accurate protein & nanoparticle size distributions
- Multimodal & unimodal size distribution software
- Size range: 0.3 nm to 10 µm depending on refractive index and concentration
- Concentration range: 0.1 ppm to 50 mg/mL, depending on sample refractive index
- Three measurement angles: 15°, 90°, and 173°
- 40 mW diode laser (nominal 640 nm wavelength)
- Temperature control from -5 °C to 110 °C ± 0.1 °C. No external circulator required.
- Precision: ± 1 %
- Accuracy: ± 2 %
Zeta potential
- Rapid zeta potential measurements for proteins, peptides, nucleic acids and other biological samples
- Compatible with organic solvents, oily or viscous media, high-salt suspensions
- Particles size: 1 nm to 100 µm, sample dependent
- Concentration range: 40 % w/v, sample dependent
- Signal processing: Electrophoretic & true Phase Analysis Light Scattering, ELS & PALS
- Precision: ± 3 %
- Accuracy: 2.53 ± 0.12 µm • cm/V • s
