Imaging

NIKON CSU-X1 Spinning Disk Confocal

Applications: Intravital Imaging, 4D Confocal, Fast Kinetics, Widefield Fluorescence, Brightfield

Excitation:

- Four lasers lines for confocal imaging: 405nm, 488nm, 561nm, 640nm.
- Four LED lines for widefield fluorescence: 385nm, 488nm, 561nm, 621nm.
- Brightfield illumination.

Emission:

- Four single chanel emission filters: 455/50nm, 525/36nm, 605/52nm, 705/72nm.
- One quad chanels filter for high speed acquisition: DAPI/FITC/TRITC/CY5.

Detection:

- Confocal: Photometrics Prime BSI Express sCMOS Camera. 2048x2048, 95fps@11-bits/43fps@16-bits.
- Widefield and Brigthfield: Nikon Digital Sight 10, Color and Monochrome mode, 25 mm FOV, 23.9 megapixel.

Objectives: 10X/0.45 air, 20X/0.75 air, 16X/0.8 water dipping, 20X/1.0 water, 60X/1.42 oil.

Located in a biosafety level 2 room that include a BSC.

Image processing and analysis: Nikon imaging software (NIS) workstation and Bitplane Imaris 9.1.2

LEICA SP8 Laser Scanning Confocal and Multiphoton

Applications: 4D Confocal and Multiphoton Imaging, Spectral imaging, FLIM, FRET, FRAP modules, SHG

Excitation:

-  Four solid state laser lines: 448 nm, 488 nm, 552 nm,
  638 nm
-  One tunable IR two-photons laser: 680-1080 nm

Detection:

-  Two internal HyDs (high sensitivity detectors)
-  Two internal PMTs
-  One transmitted light PMT
-  Two reflected light HyD
-  One brightfield detector (Dodt gradient)

Objectives: 4x to 63x (including air, oil and multi-immersion)

Environmental control: CO2, temperature, humidity.

Image processing and analysis: Leica LAS X and Bitplane Imaris 9.1.2

Perfusion system for live tissue imaging

Fly eye autofluorescence

Second Harmonic and two-photon fluorescence on human aorta

PerkinElmer High Content Microscopes

Applications: Fully automated fluorescence and brightfield imaging of microplates (fixed or live samples) and standard slides. Automated high content image analysis.

Opera Phenix Plus automated microscope:

• From 6 well to 1536 well plates and standard slides.
• 

  Perkin Elmer Opera Phenix Plus and Cell Explorer Station
  5x, 10x, 20x air objective
• 20X, 40x and 60X high numerical aperture water immersion objectives
• Widefield or confocal (lens spinning disk)
• Five laser lines (375nm, 425nm, 488nm, 561nm, 640nm)
• Transmitted light
• Fourteen emission filters for wide or narrow emission window
• Four sCMOS 16 bit camera (2160px X 2160px)
• Fast frame-rate imaging capacity (up to 105 fps)
• Internal liquid handling capacity for fast kinetic acquisition (i.e., Calcium imaging)
• Connected to a plate handler, a liquid handler, a plate washer, an incubator and a bar scanner.
• Optional environmental control chamber (CO2, Temperature, Humidity)

Operetta automated microscope:

• From 6 well to 1536 well plates and standard slides
• 

  Perkin Elmer Operetta automated microscope
  2x, 10x, 20x and 40x objectives
• A wide range of filter combinations
• Optional confocality (spinning disk)
• Optional environmental control chamber
• 300W xenon lamp

Browser-based image analysis software (Columbus)

Stereo-microscope (Leica M165)

-  Color camera 5Mp (Leica DFC450C)
-  Epifluorescence (120W Mercury Halide lamp)
-  Filter: green (GFP), red (RhodB), YFP, mCherry
-  Reflected and Transmitted light set-up
-  Leica LAS X acquisition software with multi focus and overlay module

Upright microscope (Leica DM1000)

-  Color camera 1.4Mp (Leica DFC310FX)
-  Epifluorescence, Brightfield (120W Mercury Halide lamp)
-  Filter: blue (A4), red (N2.1), green (L5)
-  Leica LAS X acquisition software

Inverted microscope (Leica DMI 6000)

-  Monochrome camera 2Mp (Leica DFC345FX)
-  Epifluorescence, DIC, IMC (120W Mercury Halide lamp)
-  Filter sets for DAPI, CFP, GFP and TxRed
-  Leica LAS X acquisition software
-  Eppendorf piezo system for microinjection/manipulation