Training Program

Technique

Description

Training Information

Blood Collections

Cardiac Puncture

Terminal procedure. See Isoflurane followed by physical method.

See Isoflurane followed by physical method.

Jugular Vein

Drawing blood from the jugular vein in the neck

Mice: Taught in specialized training, modules 1 and 3 are prerequisites.

Module 2 is required if using an injectable.

Rats: Taught in specialized training, module 1 is a prerequisite.

Retro-orbital (justify)

Drawing blood from the orbital sinus located behind the eye

Taught in specialized training, module 1 is a prerequisite.

Saphenous Vein

Drawing blood from the lateral saphenous vein in the hind leg

Taught in module 7, module 1 is a prerequisite.

Submandibular

Drawing blood from the submandibular vein, located beneath the jaw

Taught in module 7, module 1 is a prerequisite.

Tail Puncture

Collecting blood using the point of a blade/needle to puncture the vessel

Taught in specialized training, module 1 is a prerequisite.

Tail Snip

Collecting blood by transecting (cutting off) portion of mouse tail under anesthetic and analgesia. Repeat samples can be

taken by removal of the scab.

Taught in specialized training, modules 1 and 3 are prerequisites.

Tail Vein

Drawing blood from the coccygeal venipuncture in the tail

Taught in module 7, module 1 is a prerequisite.

Injections

Intradermal (Intraplantar, Footpad...)

Administered into the dermal layer of the skin

Taught in specialized training, module 1 is a prerequisite.

Intrafemoral

Administered into the femoral bone

Taught in specialized training, modules 1,2 and 3 are prerequisites.

Intramuscular

Administered into the muscular tissue

Mice: Taught in specialized training, module 1 is a prerequisite.

Rats: Taught in module 2, module 1 is a prerequisite.

Intranasal (Instillation)

Administered through the nasal passages

Requires passing module 1.

Unless performed under anesthesia, which requires module 3

Intraperitoneal

Administered into the peritoneal cavity

Taught in module 2, module 1 is a prerequisite.

Intratracheal (Instillation)

Administered directly into the trachea

Taught in specialized training, modules 1 and 3 are prerequisites.

Intratibial

Administered into the tibia (shinbone)

Taught in specialized training, module 1 is a prerequisite

Intracranial (Neonates only)

Administered into the brain

Requires modules 1, 2 and 3.

Intravenous/ Tail vein

Administered into the vein via the tail

Taught in module 6, module 1 is a prerequisite

Subcutaneous

Administered beneath the skin

Taught in module 2, module 1 is a prerequisite.

Retro-Orbital (justify)

Administered into orbital sinus behind the eye

Taught in specialized training, module 1 is a prerequisite

Via Cannulation

Administered into a surgically implanted tube

Requires module 1.

Via Tumour or Abscess

Administered either into tissue surrounding the tumour or abscess or directly into it.

Surrounding tissue: Requires modules 1 and 2 (for subcutaneous).

Direct: Requires module 1

Oral Gavage

Administered into the esophagus via mouth using a feeding tube or needle

Taught in specialized training, module 1 is a prerequisite

Other procedures

Handling and Restraint

Holding and immobilizing the animal

Behavioural Testing

Testing through using behavioural apparatus

Requires module 1

Breeding Colony Management

Mating and weaning animals

Taught in module 5 (optional), module 1 is a prerequisite

Intubation

Survival procedure: Insertion of a tube into the trachea

Taught in specialized training, modules 1 and 3 are prerequisites

Implantation

Placement of a device or substance into the body

Requires modules 1,2 and 3

Rectal Administration

Introduction of substances through the rectum

Requires module 1

Genotyping - Ear Punch

Punching a small hole in the ear of an animal

Demonstration in module 1

Genotyping – Fecal Pellet

Collection of fecal pellets

Requires module 1.

Genotyping – Tail Tip

Transecting (cutting off) a small portion of a rodent's tail

Under 21 days of age: Requires module 1.

Over 21 days of age: Requires modules 1,2 and 3.

Genotyping – Toe Tip

Transecting a small portion of a young rodent’s toe (under 7 days of age)

Taught in specialized training, module 1 is a prerequisite.

Identification – Ear Punch/Ear Notch

Punching a small hole in the ear of an animal for identification purposes

Demonstration in module 1.

Identification – Micro-tattoo

Identification of animals through tattoo with a small gauge needle or micro-tattoo

Requires passing module 1, specialized training by request.

Identification – Tattoo

Identification of animals through tattoo

Requires passing modules 1 and 3, specialized training by request.

Identification – Microchip

Identification of animals through subcutaneous implant of microchip

Requires passing modules 1,2 and 3, Optional specialized training by request.

Isoflurane and CO2 followed by

Secondary Physical Confirmation

(Decapitation, Pneumothorax, Cervical Dislocation, Cardiac Puncture)

Isoflurane inhalant anesthetic followed by CO2 euthanasia, confirmed by a secondary physical method

Taught in module 1

Isoflurane followed by exsanguination (Justify)

Isoflurane inhalant anesthetic followed by severing major vein and/or artery resulting in euthanasia by blood loss

Taught in specialized training, module 1 is a prerequisite.

Isoflurane followed by physical method (Decapitation, Cervical Dislocation, Cardiac Puncture)

Isoflurane inhalant anesthetic followed by physical method of euthanasia

Requires passing Module 1 and Module 3

Anesthetic overdose followed by a secondary physical method (Decapitation, Cervical Dislocation, Cardiac Puncture)

Isofluorane overdose followed by cervical dislocation.

Euthanasia by anesthetic or barbiturate cocktail overdose, confirmed by a secondary physical method

Requires passing modules 1 and 2 to perform. Specialized training may be required if the drug is not administered Intraperitoneally.

Inhalant anesthetic overdose requires module 3.

Bell Jar Method of euthanasia using Isoflurane followed by cervical dislocation (Justify)

A closed receptacle containing cotton or gauze soaked with

Anesthetic, followed by a physical method of euthanasia

Requires passing module 1.

CO2 chamber by a secondary physical method (Decapitation, Pneumothorax or Cervical Dislocation)

(Justify)

CO2 euthanasia confirmed by a secondary physical method

Requires passing module 1.

Cervical Dislocation (without Anesthesia)

Euthanasia by cervical dislocation

Taught in specialized training, module 1 is a prerequisite.

Decapitation (without Anesthesia)

Euthanasia by decapitation

Taught in specialized training, module 1 is a prerequisite.

Perfusion, Terminal (with Anesthesia)

Isoflurane inhalant or injectable anesthetic followed by severing the right atrium and passing fluid through the lymphatic system or blood vessels to an organ or a tissue.

Requires passing Module 1 and 3.

Module 2 is required if using injectable anesthetic

Decapitation (with Anesthesia) for neonates

Isoflurane anesthetic inhalant followed by decapitation with scissors for pups under 14 days

 Module 1 is required

Decapitation (without Anesthesia) for neonates

Decapitation with scissors for pups under 14 days

Demonstration in Module 1

Decapitation (with Hypothermia Anesthesia) for neonates

Using ice to induce hypothermic anesthesia followed by decapitation with scissors for pups under 7 days

Requires passing Module 1

Technique

Description

Training Information

Anesthesia – Inhalant

Induction and maintenance of anesthesia using an inhalant machine.

Taught in module 3, module 1 is a prerequisite and in most cases module 2.

Anesthesia – Injection

Induction and maintenance of anesthesia using an injectable cocktail.

Demonstrated in module 3, modules 1 and 2 are prerequisites.

Anesthesia – Rapid Cooling (Neonates only)

Induction and maintenance of anesthesia through hypothermia of neonatal rodents.

Requires passing module 1 to perform.

Any Surgical Procedure

Any technique listed under the Study Segments section of the AUP.

Taught in module 4 (optional). Requires modules 1,2 and 3